Asepsis means killing of all8/17/2023 Turn the air adjustment clockwise to decrease the air (a more purple flame) and counterclockwise to increase the air (a more yellow flame). Before lighting a Bunsen burner, set the air and gas adjustments to a minimal open position. The barrel of the burner turns to adjust the amount of air going into the burner. The knob underneath adjusts the amount of gas going into the burner tube. Firmly attach the hose to the tapered gas line.If you can’t seem to get your burner lit, ask your instructor or IA if the main gas control is on. It is essential that you grasp these skills before you proceed to working with actual microorganisms. Part I - Procedures for Practice "Organisms"Īfter you have practiced these procedures several times your instructor or IA will assess your proficiency. The longer your organism is exposed to the air, the more opportunities there are for it to get contaminated and/or to contaminate you, your lab partners or your equipment. It is imperative that you do this quickly and safely. You will spend a lot of time in lab transferring organisms from one tube to another, or to slides or to plates. One of your main concerns when working with microorganisms is to avoid producing aerosols that you can breathe in and droplets that can land on you, your lab partners, and your lab equipment. Each of you have your own loop and tubes, but you will be sharing a Bunsen burner and other lab equipment. Do not stretch over the table for what you need. You need to have all your materials close to you. You need to have your workspace well organized.
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